移至主內容
胡念仁 助理教授
英國蘇格蘭愛丁堡大學博士
生命科學大樓1229室
04-22840468#228
njhu@nchu.edu.tw

2013/08 -迄今       國立台中中興大學生物化學研究所 助理教授 
2010/01-2013/07  英國同步輻射中心Diamond Light Source 博士後研究員 
2007/01-2009/12  英國倫敦帝國理工學院 博士後研究員 

Na+-coupled symporters

Cation/Proton antiporters

Potassium channels

Pore-forming toxins

Nucleotide signaling pathway

實驗室簡介

Topology map determination using SDAF

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Na+-induced conformational change of ASBTNm

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

SCI Publications

1.         Lu, P. H., Li, C. C., Chiang, Y. W., Liu, J. H., Tien Chiang, W., Chao, Y. H., Li, G. S., Weng, S. E., Lin, S. Y., and *Hu, N. J. (2020) Dissecting the Conformational Dynamics of the Bile Acid Transporter Homologue ASBTNM. J Mol Biol, 166764

2.         Huang, C. W., Lin, Y. S., Huang, W. C., Lai, C. C., Chien, H. J., *Hu, N. J., and Chen, J. H. (2020) Inhibition of the clinical isolates of Acinetobacter baumannii by Pseudomonas aeruginosa: In vitro assessment of a case-based study. J Microbiol Immunol Infect (共同通訊作者)

3.         Wong, H. L., Hu, N. J., Juang, T. Y., and Liu, Y. C. (2020) Co-Immobilization of Xylanase and Scaffolding Protein onto an Immobilized Metal Ion Affinity Membrane. Catalysts 10, 1408

4.         Fang, Y. T., Li, S. Y., Hu, N. J., Yang, J., Liu, J. H., and Liu, Y. C. (2020) Study on Cecropin B2 Production via Construct Bearing Intein Oligopeptide Cleavage Variants. Molecules 25

5.         Lin, Y. H., Lin, S. Y., Li, G. S., Weng, S. E., Tzeng, S. L., Hsiao, Y. H., and *Hu, N. J. (2019) Site-Directed Alkylation Detected by In-Gel Fluorescence (SDAF) to Determine the Topology Map and Probe the Solvent Accessibility of Membrane Proteins. Sci Rep 9, 13171

6.         Liu, C. H., Chen, Y. T., Hou, M. H., Hu, N. J., Chen, C. S., and Shaw, J. F. (2018) Crystallographic analysis of the Staphylococcus epidermidis lipase involved in esterification in aqueous solution. Acta Crystallogr F Struct Biol Commun 74, 351-354

7.         Tseng, W. H., Chang, C. K., Wu, P. C., Hu, N. J., Lee, G. H., Tzeng, C. C., Neidle, S., and Hou, M. H. (2017) Induced-Fit Recognition of CCG Trinucleotide Repeats by a Nickel-Chromomycin Complex Resulting in Large-Scale DNA Deformation. Angew Chem Int Ed Engl 56, 8761-8765

8.         Huang, T. Y., Chang, C. K., Kao, Y. F., Chin, C. H., Ni, C. W., Hsu, H. Y., Hu, N. J., Hsieh, L. C., Chou, S. H., Lee, I. R., and Hou, M. H. (2017) Parity-dependent hairpin configurations of repetitive DNA sequence promote slippage associated with DNA expansion. Proc Natl Acad Sci U S A 114, 9535-9540

9.         Lin, S. Y., Sun, X. H., Hsiao, Y. H., Chang, S. E., Li, G. S., and *Hu, N. J. (2016) Fluorophore Absorption Size Exclusion Chromatography (FA-SEC): An Alternative Method for High-Throughput Detergent Screening of Membrane Proteins. PloS one 11, e0157923

10.       Hou, M. H., Chuang, C. Y., Ko, T. P., Hu, N. J., Chou, C. C., Shih, Y. P., Ho, C. L., and Wang, A. H. (2016) Crystal structure of vespid phospholipase A1 reveals insights into the mechanism for cause of membrane dysfunction. Insect Biochem Mol Biol 68, 79-88

11.       Chia, J. Y., Tan, W. S., Ng, C. L., Hu, N. J., Foo, H. L., and Ho, K. L. (2016) A/T Run Geometry of B-form DNA Is Independent of Bound Methyl-CpG Binding Domain, Cytosine Methylation and Flanking Sequence. Sci Rep 6, 31210

12.       Chang, C. K., Jeyachandran, S., Hu, N. J., Liu, C. L., Lin, S. Y., Wang, Y. S., Chang, Y. M., and Hou, M. H. (2016) Structure-based virtual screening and experimental validation of the discovery of inhibitors targeted towards the human coronavirus nucleocapsid protein. Mol Biosyst 12, 59-66 (共同第一作者)

13.       Chin, K. H., Liang, J. M., Yang, J. G., Shih, M. S., Tu, Z. L., Wang, Y. C., Sun, X. H., Hu, N. J., Liang, Z. X., Dow, J. M., Ryan, R. P., and Chou, S. H. (2015) Structural Insights into the Distinct Binding Mode of Cyclic Di-AMP with SaCpaA_RCK. Biochemistry 54, 4936-4951

14.       Axford, D., Foadi, J., Hu, N. J., Choudhury, H. G., Iwata, S., Beis, K., Evans, G., and Alguel, Y. (2015) Structure determination of an integral membrane protein at room temperature from crystals in situ. Acta Crystallogr D Biol Crystallogr 71, 1228-1237 (共同第一作者)

15.       Hu, N. J., Rada, H., Rahman, N., Nettleship, J., Bird, L., Iwata, S., Drew, D., Cameron, A., and Owen, R. (2014) GFP-based expression screening of membrane proteins in insect cells using the baculovirus system. Methods Mol Biol 1261, 197-209

16.       Weeratunga, S. K., Osman, A., Hu, N. J., Wang, C. K., Mason, L., Svard, S., Hope, G., Jones, M. K., and Hofmann, A. (2012) Alpha-1 giardin is an annexin with highly unusual calcium-regulated mechanisms. J Mol Biol 423, 169-181

17.       Weeratunga, S., Hu, N. J., Simon, A., and Hofmann, A. (2012) SDAR: a practical tool for graphical analysis of two-dimensional data. BMC Bioinformatics 13, 201

18.       Sonoda, Y., Newstead, S., Hu, N. J., Alguel, Y., Nji, E., Beis, K., Yashiro, S., Lee, C., Leung, J., Cameron, A. D., Byrne, B., Iwata, S., and Drew, D. (2011) Benchmarking membrane protein detergent stability for improving throughput of high-resolution X-ray structures. Structure 19, 17-25

19.       Hu, N. J., Iwata, S., Cameron, A. D., and Drew, D. (2011) Crystal structure of a bacterial homologue of the bile acid sodium symporter ASBT. Nature 478, 408-411

20.       Hu, N. J., Yusof, A. M., Winter, A., Osman, A., Reeve, A. K., and Hofmann, A. (2008) The crystal structure of calcium-bound annexin Gh1 from Gossypium hirsutum and its implications for membrane binding mechanisms of plant annexins. J Biol Chem 283, 18314-18322

21.       Hu, N. J., Bradshaw, J., Lauter, H., Buckingham, J., Solito, E., and Hofmann, A. (2008) Membrane-induced folding and structure of membrane-bound annexin A1 N-terminal peptides: implications for annexin-induced membrane aggregation. Biophys J 94, 1773-1781

22.       Yusof, A. M., Hu, N. J., Wlodawer, A., and Hofmann, A. (2005) Structural evidence for variable oligomerization of the N-terminal domain of cyclase-associated protein (CAP). Proteins 58, 255-262

23.       Hu, N. J., and Hofmann, A. (2005) Automated CD and fluorescence data processing with ACDP and AFDP (v2.4). Appl. Spectrosc. 59

24.       Dabitz, N., Hu, N. J., Yusof, A. M., Tranter, N., Winter, A., Daley, M., Zschornig, O., Brisson, A., and Hofmann, A. (2005) Structural determinants for plant annexin-membrane interactions. Biochemistry 44, 16292-16300

 

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