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Nien-Jen Hu Associate Professor
PhD, University of Edinburgh, Scotland
Room 1229, Life Science Building

2022/08/01-         Chief Executive of EMI bilingual education project, College of Life Sciences, NCHU

2021/08-               Associate Professor, Institute of Biochemistry, National Chung Hsing University, Taiwan

2013/08-2021/07  Assistant Professor, Institute of Biochemistry, National Taichung Chung Hsing University

2010/01-2013/07 Postdoctoral researcher at Diamond Light Source, UK Synchrotron Radiation Center

2007/01-2009/12 Postdoctoral researcher at Imperial College London, UK

Na+-coupled symporters

Cation/Proton antiporters

Potassium channels

Pore-forming toxins

Nucleotide signaling pathway


Topology map determination using SDAF






















Na+-induced conformational change of ASBTNm




















1.         Fang, Y. T., Li, S. Y., Hu, N. J., Yang, J., Liu, J. H., and Liu, Y. C. (2020) Study on Cecropin B2 Production via Construct Bearing Intein Oligopeptide Cleavage Variants. Molecules 25

2.         Lin, Y. H., Lin, S. Y., Li, G. S., Weng, S. E., Tzeng, S. L., Hsiao, Y. H., and Hu, N. J. (2019) Site-Directed Alkylation Detected by In-Gel Fluorescence (SDAF) to Determine the Topology Map and Probe the Solvent Accessibility of Membrane Proteins. Sci Rep 9, 13171

3.         Liu, C. H., Chen, Y. T., Hou, M. H., Hu, N. J., Chen, C. S., and Shaw, J. F. (2018) Crystallographic analysis of the Staphylococcus epidermidis lipase involved in esterification in aqueous solution. Acta Crystallogr F Struct Biol Commun 74, 351-354

4.         Tseng, W. H., Chang, C. K., Wu, P. C., Hu, N. J., Lee, G. H., Tzeng, C. C., Neidle, S., and Hou, M. H. (2017) Induced-Fit Recognition of CCG Trinucleotide Repeats by a Nickel-Chromomycin Complex Resulting in Large-Scale DNA Deformation. Angew Chem Int Ed Engl 56, 8761-8765

5.         Huang, T. Y., Chang, C. K., Kao, Y. F., Chin, C. H., Ni, C. W., Hsu, H. Y., Hu, N. J., Hsieh, L. C., Chou, S. H., Lee, I. R., and Hou, M. H. (2017) Parity-dependent hairpin configurations of repetitive DNA sequence promote slippage associated with DNA expansion. Proc Natl Acad Sci U S A 114, 9535-9540

6.         Lin, S.-Y., Sun, X.-H., Hsiao, Y.-H., Chang, S.-E., Li, G.-S., and Hu, N.-J. (2016) Fluorophore Absorption Size Exclusion Chromatography (FA-SEC): An Alternative Method for High-Throughput Detergent Screening of Membrane Proteins. PloS one 11, e0157923

7.         Hou, M. H., Chuang, C. Y., Ko, T. P., Hu, N. J., Chou, C. C., Shih, Y. P., Ho, C. L., and Wang, A. H. (2016) Crystal structure of vespid phospholipase A1 reveals insights into the mechanism for cause of membrane dysfunction. Insect Biochem Mol Biol 68, 79-88

8.         Chia, J. Y., Tan, W. S., Ng, C. L., Hu, N. J., Foo, H. L., and Ho, K. L. (2016) A/T Run Geometry of B-form DNA Is Independent of Bound Methyl-CpG Binding Domain, Cytosine Methylation and Flanking Sequence. Sci Rep 6, 31210

9.         Chang, C. K., Jeyachandran, S., Hu, N. J., Liu, C. L., Lin, S. Y., Wang, Y. S., Chang, Y. M., and Hou, M. H. (2016) Structure-based virtual screening and experimental validation of the discovery of inhibitors targeted towards the human coronavirus nucleocapsid protein. Mol Biosyst 12, 59-66

10.       Chin, K. H., Liang, J. M., Yang, J. G., Shih, M. S., Tu, Z. L., Wang, Y. C., Sun, X. H., Hu, N. J., Liang, Z. X., Dow, J. M., Ryan, R. P., and Chou, S. H. (2015) Structural Insights into the Distinct Binding Mode of Cyclic Di-AMP with SaCpaA_RCK. Biochemistry 54, 4936-4951

11.       Axford, D., Foadi, J., Hu, N. J., Choudhury, H. G., Iwata, S., Beis, K., Evans, G., and Alguel, Y. (2015) Structure determination of an integral membrane protein at room temperature from crystals in situ. Acta Crystallogr D Biol Crystallogr 71, 1228-1237

12.       Hu, N. J., Rada, H., Rahman, N., Nettleship, J., Bird, L., Iwata, S., Drew, D., Cameron, A., and Owen, R. (2014) GFP-based expression screening of membrane proteins in insect cells using the baculovirus system. Methods Mol Biol 1261, 197-209

13.       Weeratunga, S. K., Osman, A., Hu, N. J., Wang, C. K., Mason, L., Svard, S., Hope, G., Jones, M. K., and Hofmann, A. (2012) Alpha-1 giardin is an annexin with highly unusual calcium-regulated mechanisms. J Mol Biol 423, 169-181

14.       Weeratunga, S., Hu, N. J., Simon, A., and Hofmann, A. (2012) SDAR: a practical tool for graphical analysis of two-dimensional data. BMC Bioinformatics 13, 201

15.       Sonoda, Y., Newstead, S., Hu, N. J., Alguel, Y., Nji, E., Beis, K., Yashiro, S., Lee, C., Leung, J., Cameron, A. D., Byrne, B., Iwata, S., and Drew, D. (2011) Benchmarking membrane protein detergent stability for improving throughput of high-resolution X-ray structures. Structure 19, 17-25

16.       Hu, N. J., Iwata, S., Cameron, A. D., and Drew, D. (2011) Crystal structure of a bacterial homologue of the bile acid sodium symporter ASBT. Nature 478, 408-411

17.       Hu, N. J., Yusof, A. M., Winter, A., Osman, A., Reeve, A. K., and Hofmann, A. (2008) The crystal structure of calcium-bound annexin Gh1 from Gossypium hirsutum and its implications for membrane binding mechanisms of plant annexins. J Biol Chem 283, 18314-18322

18.       Hu, N. J., Bradshaw, J., Lauter, H., Buckingham, J., Solito, E., and Hofmann, A. (2008) Membrane-induced folding and structure of membrane-bound annexin A1 N-terminal peptides: implications for annexin-induced membrane aggregation. Biophys J 94, 1773-1781

19.       Yusof, A. M., Hu, N. J., Wlodawer, A., and Hofmann, A. (2005) Structural evidence for variable oligomerization of the N-terminal domain of cyclase-associated protein (CAP). Proteins 58, 255-262

20.       Hu, N. J., and Hofmann, A. (2005) Automated CD and fluorescence data processing with ACDP and AFDP (v2.4). Appl. Spectrosc. 59

21.       Dabitz, N., Hu, N. J., Yusof, A. M., Tranter, N., Winter, A., Daley, M., Zschornig, O., Brisson, A., and Hofmann, A. (2005) Structural determinants for plant annexin-membrane interactions. Biochemistry 44, 16292-16300